Calibration

The use of a separate Reference Electrode such as our 8-702 is required.

These electrodes are standardized in two pH buffers.  Follow the procedure recommended by the manufacturer of your pH meter for calibrating your pH meter with our 8-705 or 16-705.

Electrode Setup:  Attach the proper size tubing to each end of the flow-thru "T" (1/8" I.D.).  If you are using the 8-702 Flow-thru Reference Electrode with the 8-705, also attach this reference electrode in line.  A syringe or peristaltic pump can be used to draw buffers into the electrode chambers.

Optimum Response Time:  Optimum response time will be obtained after the probe has been exercised in two buffer solutions.  Place a pH 4.01 buffer or equivalent in a beaker and a pH 6.86 buffer or equivalent in a second beaker.  Using the syringe or peristaltic pump, draw some of the pH 4.01 buffer through the electrodes allowing 5 seconds for equilibration.  Now draw the pH 6.86 buffer into the electrode chambers in the same manner. Do this several times.

Handling

Although the electrode appears to be indestructible, always handle the electrode with the same care you would use with other glass electrodes..

Cleaning

When using the electrodes in solutions containing protein, the electrode should be rinsed through with an enzyme cleaning solution such as Terg-a-zyme (Alconox, Inc.) or a chromic/sulfuric glass cleaning solution after each use for a couple of minutes to remove the protein from the glass and the reference junction.  This will prolong the useful life of the electrodes.

Storing

Always clean the electrodes before storing:

Long- term (over 2 weeks):  Return the electrode to its original container and prepare it in the same condition is which you received it.  Usually this means simply rinsing the chamber with distilled water and blow drying the chamber to remove the water.

Short-term:  The electrode chamber can be filled with an acid pH buffer solution,
e.g. pH 4.01, and the ends capped with the proper size tubing.