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MI-410, MI-412, MI-415 and
MI-710
Micro-combination
pH Microelectrodes
Operating
Instructions |
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The microelectrode is ready to use.
Carefully unwind the tape and remove
the probe from the protective glass
tube. The white translucent sleeve,
which covers the hole, should be
moved down the glass barrel slightly
to ventilate the reference chamber
and replaced after each use.
The use of a
separate Reference Electrode is not
required.
Calibration
The
probe can be held by hand when
measuring pH. It has been
standardized in two (2) pH buffers.
Follow the procedure recommended by
the manufacturer of your pH meter
for calibrating your pH meter with
our microelectrode.
Optimum Response Time:
Optimum response time will be
obtained after the probe has been
exercised in two (2) buffer
solutions. Fill one beaker with a
pH 4 buffer or equivalent and a
second beaker with a pH 7 buffer or
equivalent. Hold the microelectrode
and touch the pH 4 buffer surface
allowing 5 seconds for
equilibration, and then touch the pH
7 buffer surface in the same
manner. Do this several times. It
is not necessary to rinse the probe
because the carry-over is
negligible.
Handling
The
outer reference chamber of the
microelectrode can be refilled when
necessary with the 3 M KCl
solution, using the bulb pipette.
The reference chamber should be
filled slowly.
Be careful
not to apply pressure against the
inner glass capillary tube.
Cleaning
When using the
microelectrode in solutions
containing protein, it should be
soaked in an enzyme cleaning
solution such as Terg-a-zyme
(Alconox,
Inc.) or a
chromic/sulfuric acid glass cleaning
solution for a couple of minutes
after each use to remove the protein
from the glass and the reference
junction. This will prolong
the useful life of the
microelectrode.
Storage
Always clean the microelectrode
before storing:
Long-term (over 2 weeks):
Return the probe to its original
container and prepare it in the same
condition in which you received it.
Usually this means simply moistening
the sponge located in the bottom of
the protective glass tube with pH 4
buffer.
Short-term: The
probe can be left in an acid pH
buffer solution (pH 4.01).
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